Journal: The Journal of biological chemistry

Article Title: The pro-atherogenic cytokine interleukin-18 induces CXCL16 expression in rat aortic smooth muscle cells via MyD88, interleukin-1 receptor-associated kinase, tumor necrosis factor receptor-associated factor 6, c-Src, phosphatidylinositol 3-kinase, Akt, c-Jun N-terminal kinase, and activator protein-1 signaling.

doi: 10.1074/jbc.M502586200

Figure Lengend Snippet: FIG. 11. IL-18 induced CXCL16 expression through Src and JNK. A, IL-18 increased AP-1-dependent reporter gene activity via Src and JNK. ASMC were transfected with pAP-1-Luc and pRL-TK and after 24 h treated with the Src kinase inhibitors PP1 and PP2, the JNK inhibitor SP600125, or antisense JNK oligonucleotides followed by 25 ng/ml IL-18 for 7 h. Reporter gene assays were carried out as before. The results are the mean S.E. of four experiments. *, p 0.001 (versus untreated); †, p 0.05 versus IL-18. DMSO, Me2SO. B, IL-18 induced CXCL16 promoter-reporter activity via Src and JNK. Experimental conditions were as in A, except that the S2 construct was used instead of pAP-1-Luc. The results are the mean S.E. of six experiments. *, p 0.001 (versus untreated); †, p 0.05 versus IL-18. C, IL-18 induced CXCL16 mRNA expression via Src and JNK. Experimental conditions were as in A, except that the cells were treated with 20 ng/ml IL-18 for 2 h. Northern analysis was carried out as before. D, SP600125 inhibited the IL-18-induced phosphorylation of c-Jun Ser63 and c-Jun Ser73 in ASMC. To confirm that the dose of JNK inhibitor used in A inhibited the IL-18 activation of c-Jun, ASMC were treated with 20 M SP600125 for 30 min, then 25 ng/ml IL-18 for 1 h, and whole cell lysates were assayed for total c-Jun, c-Jun Ser63, and c-Jun Ser73 by Western blot. -Actin was used as the cell loading control.

Article Snippet: Antibodies to c-Src (sc-5266), p-c-Src Tyr530 (sc-16846), p-c-Src Tyr139 sc-12928-R), p-c-Jun Ser63 (sc7980-R), p-c-Jun Ser73 (sc-16311-R), MyD88 (sc-8197), and actin were all obtained from Santa Cruz Biotechnology.

Techniques: Expressing, Activity Assay, Transfection, Construct, Northern Blot, Phospho-proteomics, Activation Assay, Western Blot, Control

Journal: Cell reports

Article Title: iRQC, a surveillance pathway for 40S ribosomal quality control during mRNA translation initiation

doi: 10.1016/j.celrep.2021.109642

Figure Lengend Snippet: RESOURCE TABLE

Article Snippet: Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-RPS2 (uS5) Bethyl Laboratories Cat# A303-794A; RRID:AB_11218192 Rabbit monoclonal anti-RPS3 (uS3) Bethyl Laboratories Cat# A303-840A; RRID:AB_2615588 Rabbit polyclonal anti-RPS10 (eS10) ABclonal Cat# A6056; RRID:AB_2766730 Rabbit monoclonal anti-RPS20 (uS10_ Abcam Cat# ab133776; RRID:AB_2714148 Rabbit monoclonal anti-p-eIF2 α Cell Signaling Technology Cat# 3398S; RRID:AB_2096481 Rabbit polyclonal anti-RPL7 (uL30) Bethyl Laboratories Cat# A300-741A; RRID:AB_2301241 Rabbit polyclonal anti-USP10 Abcam Cat# ab72486; RRID:AB_1271412 Mouse monoclonal α-Tubulin Cell Signaling Technology Cat# 3873; RRID:AB_1904178 Mouse monoclonal c-Myc Santa Cruz Biotechnology Cat# sc-40 AC; RRID:AB_2857941 Mouse monoclonal anti-tubulin Cell Signaling Technology Cat# 3873S; RRID:AB_1904178 Anti-Rabbit IgG (H+L), HRP Conjugate antibody Promega Cat# W4011; RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate antibody Promega Cat# W4021; RRID:AB_430834 Lipofectamine RNAiMax Thermo-Fisher Cat# 13778030 Lipofectamine 2000 THermo-Fisher Cat# 11668019 Mirus TransIT 293 Mirus Bio llc Cat# MIR 2700 Protease inhibitor cocktail tablet Roche Cat# 11836170001 Immun-Blot® PVDF Membrane BioRad Cat# 1620177 Albumin Standard Thermo Scientific Cat# 23209 Clarity™ Western ECL Substrate BioRad Cat# 170-5061 Chemicals DL-Dithiothreitol ACROS organics Cat# 165680050 Anisomycin Fisher Scientific Cat# 50995788 Harringtonine LKT labs Cat# H0169 Thapsigargin Tocris Biosciences Cat# 1138 Cycloheximide MP Biomedicals Cat# 100183 Sodium arsenite solution Fluka Analytical Cat# 35000-1L-R Torin1 Tocris Biosciences Cat# 4247 PatamineA Gift from Jeremy Pelletier’s lab MG-132 Enzo Life Sciences Cat# BML-PI102 Rocaglamide MedChemExpress Cat# HY19356 Lactimidomycin Millipore Sigma Cat# 5062910001 Bafilomycin A Sigma Cat# B1793 SAR405 APExBio Cat# A8883 Doxycycline hydrochloride Fisher Scientific Cat# BP2653-5 Ammonium persulfate (≥ 98%) Sigma Cat# A3678 Fetal Bovine Serum VWR Cat# 97068-085 Trypsin Sigma-Aldrich Cat# T1426 N-Ethylmaleimide Sigma-Aldrich Cat# E3876 Puromycin Mediatech (Corning) Cat# 61-385-RA RNaseA Thermo Scientific Cat# EN0531 SUPERase-In™ Invitrogen Cat# AM2696 Turbo™ DNase Invitrogen Cat# AM2238 Trizol Life Technologies Cat# 15596026 Dimethyl sulfoxide Corning Cat# 25-950-CQC β-mercaptoethanol J.T.

Techniques: Protease Inhibitor, Western Blot, Bicinchoninic Acid Protein Assay, Bradford Assay, Recombinant, Sequencing, Software

Journal: Cell reports

Article Title: iRQC, a surveillance pathway for 40S ribosomal quality control during mRNA translation initiation

doi: 10.1016/j.celrep.2021.109642

Figure Lengend Snippet: RESOURCE TABLE

Article Snippet: Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-RPS2 (uS5) Bethyl Laboratories Cat# A303-794A; RRID:AB_11218192 Rabbit monoclonal anti-RPS3 (uS3) Bethyl Laboratories Cat# A303-840A; RRID:AB_2615588 Rabbit polyclonal anti-RPS10 (eS10) ABclonal Cat# A6056; RRID:AB_2766730 Rabbit monoclonal anti-RPS20 (uS10_ Abcam Cat# ab133776; RRID:AB_2714148 Rabbit monoclonal anti-p-eIF2 α Cell Signaling Technology Cat# 3398S; RRID:AB_2096481 Rabbit polyclonal anti-RPL7 (uL30) Bethyl Laboratories Cat# A300-741A; RRID:AB_2301241 Rabbit polyclonal anti-USP10 Abcam Cat# ab72486; RRID:AB_1271412 Mouse monoclonal α-Tubulin Cell Signaling Technology Cat# 3873; RRID:AB_1904178 Mouse monoclonal c-Myc Santa Cruz Biotechnology Cat# sc-40 AC; RRID:AB_2857941 Mouse monoclonal anti-tubulin Cell Signaling Technology Cat# 3873S; RRID:AB_1904178 Anti-Rabbit IgG (H+L), HRP Conjugate antibody Promega Cat# W4011; RRID:AB_430833 Anti-Mouse IgG (H+L), HRP Conjugate antibody Promega Cat# W4021; RRID:AB_430834 Lipofectamine RNAiMax Thermo-Fisher Cat# 13778030 Lipofectamine 2000 THermo-Fisher Cat# 11668019 Mirus TransIT 293 Mirus Bio llc Cat# MIR 2700 Protease inhibitor cocktail tablet Roche Cat# 11836170001 Immun-Blot® PVDF Membrane BioRad Cat# 1620177 Albumin Standard Thermo Scientific Cat# 23209 Clarity™ Western ECL Substrate BioRad Cat# 170-5061 Chemicals DL-Dithiothreitol ACROS organics Cat# 165680050 Anisomycin Fisher Scientific Cat# 50995788 Harringtonine LKT labs Cat# H0169 Thapsigargin Tocris Biosciences Cat# 1138 Cycloheximide MP Biomedicals Cat# 100183 Sodium arsenite solution Fluka Analytical Cat# 35000-1L-R Torin1 Tocris Biosciences Cat# 4247 PatamineA Gift from Jeremy Pelletier’s lab MG-132 Enzo Life Sciences Cat# BML-PI102 Rocaglamide MedChemExpress Cat# HY19356 Lactimidomycin Millipore Sigma Cat# 5062910001 Bafilomycin A Sigma Cat# B1793 SAR405 APExBio Cat# A8883 Doxycycline hydrochloride Fisher Scientific Cat# BP2653-5 Ammonium persulfate (≥ 98%) Sigma Cat# A3678 Fetal Bovine Serum VWR Cat# 97068-085 Trypsin Sigma-Aldrich Cat# T1426 N-Ethylmaleimide Sigma-Aldrich Cat# E3876 Puromycin Mediatech (Corning) Cat# 61-385-RA RNaseA Thermo Scientific Cat# EN0531 SUPERase-In™ Invitrogen Cat# AM2696 Turbo™ DNase Invitrogen Cat# AM2238 Trizol Life Technologies Cat# 15596026 Dimethyl sulfoxide Corning Cat# 25-950-CQC β-mercaptoethanol J.T.

Techniques: Protease Inhibitor, Western Blot, Bicinchoninic Acid Protein Assay, Bradford Assay, Recombinant, Sequencing, Software

Journal: BMC Medicine

Article Title: Pinocembrin protects against β-amyloid-induced toxicity in neurons through inhibiting receptor for advanced glycation end products (RAGE)-independent signaling pathways and regulating mitochondrion-mediated apoptosis

doi: 10.1186/1741-7015-10-105

Figure Lengend Snippet: Primary antibodies and secondary antibodies used in this study

Article Snippet: Phospho-c-Jun (Ser73) mouse mAb , 1:100 , CST , Alexa Fluor 555 donkey anti-mouse (1:500, Invitrogen, Carlsbad, CA, USA).

Techniques: